Antibodies are a special type of Y-shaped protein that is indispensable for scientific research. Western blot (WB), immunoprecipitation (IP), immunohistochemistry (IHC), immunofluorescence (IF), chromatin immunoprecipitation (ChIP), flow cytometry (FC), and many other experiments all involve the use of antibodies.
So what is the difference between the antibodies used in the different experiments? If the test results are unsatisfactory, ask yourself whether you have chosen the right antibody. To get the best antibody products, you can also check out Boster Bio featured products online.
Some suggestions for choosing the right antibody are:
1. Specialty options
When choosing specificity, there are basically four aspects to consider: protein specificity, species specificity, experimental method specificity, and label specificity.
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Each individual antibody protein can specifically bind to a unique antigen epitope due to the unique antigen-binding site at the end of the Y-shaped protein variable region of the antibody. This specificity allows precise detection of the target antigen.
2. Choice of antibody depends on experiment/application
Many people suggest that you use a monoclonal antibody as the primary antibody in WB. Currently, the technology for producing polyclonal antibodies is as mature as the technology for producing monoclonal antibodies.
Therefore, many polyclonal antibodies also performed well in WB. In addition to primary antibodies and secondary antibodies, WB involves the use of control antibodies for loading, thereby ensuring that the loading of proteins in the gel is the same.
3. How to choose a primary antibody?
Confirm antibody name. Full names, abbreviations, alternative names, isotypes, and other details should be considered. Confirm the trial/application type. In general, antibody data sheets list the applications in which antibodies will be validated.